Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples

We report the development of a novel detection and typing methodology for h uman papillomaviruses (HPV) based on real-time PCR with the self-probing fl uorescent primers known as Scorpions. This technique is quick, el simple, s pecific, sensitive, and capable of estimating viral load per cell. We repor t the results of over 100 typing reactions performed on cell lines, biopsie s, and cervical cytobrush samples which, when compared to the current refer ence HPV detection and typing technique, present a kappa value of 0.89. We further report preliminary data suggesting a relationship between viral loa d per cell and grade of cervical disease.