A. Schober et al., Expression of growth differentiation factor-15/macrophage inhibitory cytokine-1 (GDF-15/MIC-1) in the perinatal, adult, and injured rat brain, J COMP NEUR, 439(1), 2001, pp. 32-45
We and others have recently cloned a new member of the transforming growth
factor-fi superfamily, growth differentiation factor-15/ macrophage inhibit
ory cytokine-1 (GDF-15/MIC-1). Using in situ hybridization and immunohistoc
hemistry, we determined the distribution of GDF-15/MIC-1 mRNA and protein i
n the perinatal and cryolesioned adult rat brain. The choroid plexus epithe
lium of all ventricles represents the site of strongest and almost exclusiv
e mRNA expression in the normal perinatal and adult brain. The newborn rat
brain reveals GDF-15/MIC-1 immunoreactivity (ir) in ependymal cells lining
the ventricles, in the striatal subventricular zone, and in populations of
nonneural cells of the thalamic/hippocampal lamina affixa, in addition to t
hat in the choroid plexus. Unilateral cryogenic cortical lesioning induced
a significant increase of GDF-15/MIC-1 mRNA expression and ir at the lesion
site and expression in presumed neurons within the dorsal thalamic area. A
t the lesion site, GDF-15/MIC-1-producing cells showed immuncytochemical fe
atures of neurons, macrophages, and activated microglial cells. Flourescent
microscopy revealed both intra- and extracellular GDF-15/MIC-1 ir. Up-regu
lation of GDF-15/MIC-1 in activated macrophages (M phi) is also supported b
y RT-PCR, ICC, and Western blot experiments showing pronounced induction of
GDF-15/MIC-1 expression (mRNA and protein) in retinoic acid/phorbol ester-
stimulated human M phi. Our data suggest that 1) GDF-15/MIC-1 is secreted i
nto the cerebrospinal fluid and 2) in the newborn brain may penetrate throu
gh the ependymal lining and act on developing neurons and/or glial cells. A
s a constituent of cells in the lamina affixa, the protein might be involve
d in the regulation of mesenchyme-epithelial interactions. Finally, GDF-15/
MIC-1 may also act within the antiinflammatory cytokine network activated i
n CNS lesions. (C) 2001 Wiley-Liss, Inc.