Fuc-TVII is required for T helper 1 and T cytotoxic 1 lymphocyte selectin ligand expression and recruitment in inflammation, and together with Fuc-TIV regulates naive T cell trafficking to lymph nodes
G. Smithson et al., Fuc-TVII is required for T helper 1 and T cytotoxic 1 lymphocyte selectin ligand expression and recruitment in inflammation, and together with Fuc-TIV regulates naive T cell trafficking to lymph nodes, J EXP MED, 194(5), 2001, pp. 601-614
To determine hose the alpha (1,3)fucosyltransferases Fuc-TIV and Fuc-TVII,
and the selectin ligands they control may contribute to the adaptive immune
response, contact hypersensitivity (CHS) leas characterized in mice defici
ent in either or both enzymes. We find a substantial CHS deficiency in Fuc-
TVII-/- mice, and a complete deficiency in Fuc-TIV-/-/Fuc-TVII-/- mice. The
se defects are not accounted for by alterations in the number or function o
f epidermal Langerhans cells required fur cutaneous antigen processing and
presentation. By contrast, defective CHS in Fuc-TVII-/- mice or Fuc-TIV-/-/
Fuc-TVII-/- mice is attributed in part to prominent, or nearly complete def
iciencies, respectively, in the complement of naive T lymphocytes available
in lymph nodes for antigen-dependent activation, expansion, differentiatio
n, and dissemination. Fuc-TVII deficiency also deletes expression of E- and
P-selectin ligands by Th1 and T cytotoxic 1 (Tc1) lymphocytes, annuls T ce
ll trafficking to inflamed cutaneous sites in vivo, and thereby controls an
essential component of the efferent phase of the cutaneous immune response
. These observations indicate that collaborative contributions of Fuc-TIV a
nd Fuc-TVII to L-selectin ligand synthesis, and to lymphocyte recruitment,
are requisite components of the primary cellular immune response, and assig
n an essential role to Fuc-TVII in control of E- and P-selectin ligand expr
ession by Th1 and Tc1 lymphocytes.