Non-sex-linked, nuclear cleaved amplified polymorphic sequences in Silene latifolia

Cleaved amplified polymorphic sequences (CAPS) were identified for five nuc lear genes in Silene latifolia. By using published cDNA sequences of S. lat ifolia, pairs of primers were designed to amplify small regions of six nucl ear genes. Targeted regions were successfully amplified, two of which inclu ded introns. By using direct sequencing of diploid individuals, Suitable po lymorphic sites for CAPS markers were rapidly detected in five of six of th ese gene regions, thus avoiding the tedious screening of a large panel of r estriction enzymes. Using controlled progenies, we have also shown that all these CAPS markers segregated independently of the sex phenotype, thus dem onstrating that the genes analyzed here are not located in the nonrecombini ng region of the sex chromosomes.