Effect of recombinant human granulocyte/macrophage colony-stimulating factor on the healing of colonic anastomosis in rats

The purpose of this study was to investigate the efficacy of recombinant hu man granulocyte/macrophage colony-stimulating factor (rHuGM-CSF) on the wou nd healing of colonic anastomosis in rats. In total, 40 male Wistar rats we re taken into this study. The control group (n = 20) received subcutaneous saline injection. The experiment group (n = 20) received rHuGM-CSF at 100 m ug/kg subcutaneously. Both groups underwent colonic anastomosis 2 days late r. In each group, burst pressure and hydroxyproline levels were determined 3 and 7 days after anastomosis. White blood cell (WBC) and red blood cell ( RBC) counts were determined in each group to evaluate the effect of rHuGM-C SF on hematologic parameters in rats. There was no significant difference b etween the two groups in regard to burst pressure and hydroxyproline levels ( p >.05). As expected, WBC counts significantly increased in the experime nt group on days 3 (p =.035) and 7 (p =.046) following surgery. In conclusi on, rHuGM-CSF administration and increased WBC counts did not have any posi tive effect on the wound healing of colonic anastomosis.