Simultaneous liquid chromatographic determination of Indinavir Saquinavir,and Ritonavir in human plasma with combined ultraviolet absorbance and electrochemical detection

A method is described for the simultaneous determination of protease inhibi tors Indinavir, Saquinavir, and Ritonavir in human plasma using liquid chro matography with ultraviolet and electrochemical detection, Sample pre-treat ment consisted of solid-phase extraction prior to ion-pair reversed phase h igh performance liquid chromatography with ultraviolet detection at 240 nm (Saquinavir and Ritonavir) and electrochemical detection at + 750 mV (Indin avir and Saquinavir). The method was validated with respect to specificity, linearity, limits of detection and quantification, precision, and accuracy . The proposed LC assay was utilized to analyze plasma samples from patient s treated with HIV-protease inhibitors.