Utilization of chicory roots for microbial endoinulinase production

Aims: The optimal culture conditions for endoinulinase production using chi cory roots were studied in shake-flask culture. Methods and Results: Much higher enzyme production was achieved with Xantho monas sp. (15 U ml(-1)) than with Pseudomonas sp. (3 U ml(-1)). Optimized c ulture conditions of Xanthomonas sp. for endoinulinase production in flask culture were: chicory powder, 5 g l(-1); temperature, 37 degreesC; pH, 7.0; agitation speed, 100 rev min(-1). Conclusions: Maximum bacterial growth and enzyme production were 6.2 g l(-1 ) and 20 U ml(-1) under optimal conditions, respectively. Significance and Impact of the Study: Chicory roots could be used as a ferm entation substrate for the production of enndoinulinase.