Purpose: This study was designed to compare the accuracy and bias in estima
tes of total body density (Db) by hydrostatic weighing (HW) and the BOD POD
, and percent body fat (%fat) by the BOD POD with the four-compartment mode
l (4C model) in 42 adult females. Furthermore, the role of the aqueous and
mineral fractions in the estimation of body fat by the BOD POD was examined
. Methods: Total body water was determined by isotope dilution ((H2O)-H-2)
and bone mineral was determined by dual-energy x-ray absorptiometry. Db and
%fat were determined by the BOD POD and HW. The 4C model of Baumgartner wa
s used as the criterion measure of body fat. Results: MV Db (1.0352 g.cm(-3
)) was not statistically different (P = 0.35) from BOD POD Db (1.0349 g.cm(
-3)). The regression between Db by HW and the BOD POD significantly deviate
d from the line of identity (Db by HW = 0.90 X Db by BOD POD + 0.099; R-2 =
0.94). BOD POD %fat (28.8%) was significantly lower (P < 0.01) than %fat b
y the 4C model (30.6%). The regression between %fat by the 4C model and the
BOD POD significantly deviated from the line of identity (%fat by 4C model
= 0.88 X %fat by BOD POD + 5.41%; R-2 = 0.92). BOD POD Db and %fat showed
no bias across the range of fatness. Only the aqueous fraction of the fat-f
ree mass (FFM) had a significant correlation with the difference in %fat be
tween the 4C model and the BOD POD. Conclusion: These data indicate that th
e BOD POD underpredicted body fat as compared with the 4C model, and the aq
ueous fraction of the FFM had a significant effect on estimates of %fat by
the BOD POD.