Single-molecule observation of protein-protein interactions in the chaperonin system

Authors
Taguchi, H Ueno, T Tadakuma, H Yoshida, M Funatsu, T
Citation
H. Taguchi et al., Single-molecule observation of protein-protein interactions in the chaperonin system, NAT BIOTECH, 19(9), 2001, pp. 861-865
Citations number
37
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
NATURE BIOTECHNOLOGY
ISSN journal
10870156 → ACNP
Volume
19
Issue
9
Year of publication
2001
Pages
861 - 865
Database
ISI
SICI code
1087-0156(200109)19:9<861:SOOPII>2.0.ZU;2-W
Abstract
We have analyzed the dynamics of the chaperonin (GroEL)-cochaperonin (GroES ) interaction at the single-molecule level. In the presence of ATP and non- native protein, binding of GroES to the immobilized GroEL occurred at a rat e that is consistent with bulk kinetics measurements. However, the release of GroES from GroEL occurred after a lag period (similar to3 s) that was no t recognized in earlier bulk-phase studies. This observation suggests a new kinetic intermediate in the GroEL-GroES reaction pathway.