Differential transcription factor expression in human mononuclear cells inresponse to amphotericin B: Identification with complementary DNA microarray technology
Jd. Cleary et al., Differential transcription factor expression in human mononuclear cells inresponse to amphotericin B: Identification with complementary DNA microarray technology, PHARMACOTHE, 21(9), 2001, pp. 1046-1054
Study Objective. To identify genes differentially expressed in human monocy
tic cells exposed to amphotericin B in vitro.
Design. In vitro experiment.
Setting. Hospital laboratory.
Material. Human mononuclear cell line, THP-1.
Intervention. Human mononuclear cells were exposed to amphotericin B or med
ia alone for 6 hours. After exposure, total RNA was isolated and reverse tr
anscribed to complementary DNA. Differences in probe hybridization observed
during blotting were measured, and genes with altered regulation were desc
ribed by using human complementary DNA microarray technology.
Measurements and Main Results. Of 588 genes represented on the array, 16 tr
anscripts were found to be upregulated and 4 transcripts were downregulated
in response to amphotericin B. These findings suggest that amphotericin B
alters the expression of genes in human monocytic cells that play a role in
many cellular functions, including immune response, signal transduction, a
nd cell differentiation.
Conclusion. Amphotericin B induces alterations in human cell gene transcrip
tion. These changes could be used to evaluate differences in toxicity or ef
ficacy observed in patients receiving this agent.