Differential transcription factor expression in human mononuclear cells inresponse to amphotericin B: Identification with complementary DNA microarray technology

Citation
Jd. Cleary et al., Differential transcription factor expression in human mononuclear cells inresponse to amphotericin B: Identification with complementary DNA microarray technology, PHARMACOTHE, 21(9), 2001, pp. 1046-1054
Citations number
49
Categorie Soggetti
Pharmacology
Journal title
PHARMACOTHERAPY
ISSN journal
02770008 → ACNP
Volume
21
Issue
9
Year of publication
2001
Pages
1046 - 1054
Database
ISI
SICI code
0277-0008(200109)21:9<1046:DTFEIH>2.0.ZU;2-A
Abstract
Study Objective. To identify genes differentially expressed in human monocy tic cells exposed to amphotericin B in vitro. Design. In vitro experiment. Setting. Hospital laboratory. Material. Human mononuclear cell line, THP-1. Intervention. Human mononuclear cells were exposed to amphotericin B or med ia alone for 6 hours. After exposure, total RNA was isolated and reverse tr anscribed to complementary DNA. Differences in probe hybridization observed during blotting were measured, and genes with altered regulation were desc ribed by using human complementary DNA microarray technology. Measurements and Main Results. Of 588 genes represented on the array, 16 tr anscripts were found to be upregulated and 4 transcripts were downregulated in response to amphotericin B. These findings suggest that amphotericin B alters the expression of genes in human monocytic cells that play a role in many cellular functions, including immune response, signal transduction, a nd cell differentiation. Conclusion. Amphotericin B induces alterations in human cell gene transcrip tion. These changes could be used to evaluate differences in toxicity or ef ficacy observed in patients receiving this agent.