A SHORTENED LIFE-SPAN OF EKLF- - ADULT ERYTHROCYTES, DUE TO A DEFICIENCY OF BETA-GLOBIN CHAINS, IS AMELIORATED BY HUMAN GAMMA-GLOBIN CHAINS/

Using homologous recombination, both EKLF alleles in murine embryonic stem (ES) cells were inactivated, These EKLF-/- ES cells were capable of undergoing in vitro differentiation to form definitive erythroid co lonies that were similar in size and number to those formed by wild-ty pe ES cells, However, the EKLF-/- colonies were poorly hemoglobinized and enucleated erythrocytes in these colonies contained numerous Heinz bodies, Reverse transcriptase-polymerase chain reaction (RT-PCR) anal yses revealed that adult and embryonic globin genes were appropriately regulated, with the exception of beta h1-globin, which continued to b e expressed at a very low level. The ratio of adult beta-globin/alpha- globin mRNA in the mutant ES cells was 1/15 of that in wildtype ES cel ls. When the EKLF-/- cells were injected into blastocysts, they did no t contribute at a detectable level to the mature erythrocyte compartme nt of the chimeric animars, based on analysis of glucose phosphate iso merase-l (GPI-1) isozymes and hemoglobins that distinguish ES cell-der ived erythrocytes from host blastocyst-derived erythrocytes, In contra st, semiquantitative RT-PCR analysis of RNA from reticulocytes of the same chimeric animals suggested that the ES cell-derived reticulocytes were present at a level of 6% to 8%, This indicated that the EKLF-/- erythrocytes in adult animals must be short-lived, apparently due to t he imbalance of beta-versus alpha-globin chains, leading to the precip itation of excess alpha-globin chains to form Heinz bodies, Consistent with this hypothesis, the short life span was ameliorated by introduc tion into the EKLF-/- ES cells of a human LCR/gamma-globin gene, as ev idenced by the presence of ES cell-derived reticulocytes as well as ma ture erythrocytes in the blood of the chimeric animals. (C) 1997 by Th e American Society of Hematology.