Preparation of monospecific polyclonal antibodies against Sambucus nigra lectin related protein, a glycosylated plant protein

A simple, but highly efficient, method was developed for the purification o f monospecific antibodies against the plant glycoprotein Sambucus nigra lec tin related protein. In a first step, the antiserum is purified by affinity chromatography on a column with the immobilized antigen. To deplete the af finity-purified antiserum from aspecific cross-reacting antibodies directed against the glycan part of the glycoprotein, a second affinity chromatogra phy on an unrelated plant glycoprotein, in casu the Robinia pseudoacacia ag glutinin, is included.