Immobilized artificial membrane chromatography with mass spectrometric detection: a rapid method for screening drug-membrane interactions

A high-performance liquid chromatography (HPLC)/electrospray ionization mas s spectrometry method for measuring drug-membrane interactions was develope d using immobilized artificial membrane (IAM) fast-screening mini-columns. The HPLC mobile phase consisted of phosphate-buffered saline (i.e., 5.0 mM phosphate buffer at pH 7.4, 1.35 mM KCI, and 68.5 mM NaCl) and acetonitrile . This method facilitated the measurement of IAM retention time of over ten compounds in one experiment, significantly reducing analysis time compared with the earlier IAM-HPLC method. The particular electrospray source used demonstrated the ability to tolerate the high salt-containing nonvolatile b uffer used for retention time measurement. Copyright (C) 2001 John Wiley & Sons, Ltd.