CueO is a multi-copper oxidase that confers copper tolerance in Escherichia coli

Authors
Grass, G Rensing, C
Citation
G. Grass et C. Rensing, CueO is a multi-copper oxidase that confers copper tolerance in Escherichia coli, BIOC BIOP R, 286(5), 2001, pp. 902-908
Citations number
27
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
286
Issue
5
Year of publication
2001
Pages
902 - 908
Database
ISI
SICI code
0006-291X(20010907)286:5<902:CIAMOT>2.0.ZU;2-F
Abstract
The putative multi-copper oxidase CueO had previously been implicated in in trinsic copper resistance in Escherichia coli. In this report we showed tha t the presence of CueO in the periplasm protected alkaline phosphatase from copper-induced damage. CueO contained four. copper atoms per molecule and displayed spectroscopic properties typical of blue copper oxidases. CueO ca talyzed the oxidation of p-phenylene-diamine (pPD), 2,6-dimethoxyphenol (DM P) and exhibited ferroxidase activity in vitro. (C) 2001 Academic Press.