We previously demonstrated that (A+T)-stretch binding protein (ATBP) and Do
rsal-related immunity factor (Dif) are required for the expression of the S
arcophaga lectin gene in SL-2 cells (Aozasa et al., Eur. J. Biochem. 268, 2
506-2511, 2001). The present study demonstrates that DmUbc9 interacts with
ATBP, and cotransfection of the DmUbc9 vector with ATBP and Dif vectors gre
atly enhances the expression of the luciferase reporter of the Sarcophaga l
ectin gene in SL-2 cells. These results suggest that sumoylation of ATBP is
involved in the expression of the Sarcophaga lectin gene in this system. (
C) 2001 Academic Press.