Interaction between ATBP and DmUbc9 in the expression of the Sarcophaga lectin gene

We previously demonstrated that (A+T)-stretch binding protein (ATBP) and Do rsal-related immunity factor (Dif) are required for the expression of the S arcophaga lectin gene in SL-2 cells (Aozasa et al., Eur. J. Biochem. 268, 2 506-2511, 2001). The present study demonstrates that DmUbc9 interacts with ATBP, and cotransfection of the DmUbc9 vector with ATBP and Dif vectors gre atly enhances the expression of the luciferase reporter of the Sarcophaga l ectin gene in SL-2 cells. These results suggest that sumoylation of ATBP is involved in the expression of the Sarcophaga lectin gene in this system. ( C) 2001 Academic Press.