FK506 (tacrolimus) is known as an inhibitor for calcineurin and is used in
numerous research fields. It is not clear whether intravenously injected FK
506 inhibits neuronal calcineurin. We measured the calcineurin activities o
f normal and postischemic rat hippocampi after intravenous injection of FK5
06 (3 mg/kg). Intravenously injected FK506 had no inhibitory effect on calc
ineurin activity in the hippocampi of normal and postischemic rats, whereas
FK506 inhibited the calcineurin in vitro (purified enzyme, hippocampal hom
ogenate, and hippocampal slice culture homogenate). Thus, it is considered
that intravenously injected FK506 does not act on intraneuronal calcineurin
and that several effects of FK506 are not due to the inhibition of neurona
l calcineurin. (C) 2001 Academic Press.