Intravenously injected FK506 failed to inhibit hippocampal calcineurin

Citation
M. Morioka et al., Intravenously injected FK506 failed to inhibit hippocampal calcineurin, BIOC BIOP R, 286(4), 2001, pp. 802-806
Citations number
33
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
286
Issue
4
Year of publication
2001
Pages
802 - 806
Database
ISI
SICI code
0006-291X(20010831)286:4<802:IIFFTI>2.0.ZU;2-G
Abstract
FK506 (tacrolimus) is known as an inhibitor for calcineurin and is used in numerous research fields. It is not clear whether intravenously injected FK 506 inhibits neuronal calcineurin. We measured the calcineurin activities o f normal and postischemic rat hippocampi after intravenous injection of FK5 06 (3 mg/kg). Intravenously injected FK506 had no inhibitory effect on calc ineurin activity in the hippocampi of normal and postischemic rats, whereas FK506 inhibited the calcineurin in vitro (purified enzyme, hippocampal hom ogenate, and hippocampal slice culture homogenate). Thus, it is considered that intravenously injected FK506 does not act on intraneuronal calcineurin and that several effects of FK506 are not due to the inhibition of neurona l calcineurin. (C) 2001 Academic Press.