Time course of virus-like particles (VLPS) double-stranded RNA accumulation in toxigenic and non-toxigenic strains of Aspergillus flavus

Authors
Silva, VN Durigon, EL Pires, MDC Lourenco, A de Faria, MJ Correa, B
Citation
Vn. Silva et al., Time course of virus-like particles (VLPS) double-stranded RNA accumulation in toxigenic and non-toxigenic strains of Aspergillus flavus, BRAZ J MICR, 32(1), 2001, pp. 56-60
Citations number
24
Categorie Soggetti
Biology
Journal title
BRAZILIAN JOURNAL OF MICROBIOLOGY
ISSN journal
15178382 → ACNP
Volume
32
Issue
1
Year of publication
2001
Pages
56 - 60
Database
ISI
SICI code
1517-8382(200101/03)32:1<56:TCOVP(>2.0.ZU;2-L
Abstract
Two stains of Aspergillus flavus, non-toxigenic NRRL 6550 and toxigenic NRR L 5940, were studied over a period of 44 days, in order to detect the prese nce of virus-like particles (VLPs) by means of electron microscopy (EM) and nucleic acids electrophoresis. Only the toxigenic strain contained VLPs, p resenting three-segmented dsRNA. An increase in VLPs number was observed du ring the exponential phase of fungal growth, up to day 12 of culture; after this, higher levels of aflatoxin production in toxigenic NRRL 5940 mycelia occurred in parallel with decreased VLPs replication.