Transgenic expression of sarcoplasmic reticulum Ca2+ ATPase modifies the transition from hypertrophy to early heart failure

To examine the contribution of sarcoplasmic reticulum Ca2+ ATPase (SERCA2a) to early heart failure, we subjected transgenic (TG) mice expressing SERCA 2a gene and wild-type (WT) mice to aortic stenosis (AS) for 7 weeks. At an early stage of hypertrophy (4-week AS), in vivo hemodynamic and echocardiog raphic indices were similar in TG and WT mice. By 7 weeks of AS, which is t he stage of early failure in this model, TG mice with AS had lower mortalit y than WT mice with AS (6.7% versus 29%). The magnitude of left ventricular (LV) hypertrophy was similar in WT and TG 7-week AS mice. In vivo LV systo lic function was higher in TG than in WT 7-week AS mice. In LV myocytes loa ded with fluo-3, fractional cell shortening and the amplitude of the [Ca2+] (i) transients were higher in TG than in WT 7-week AS mice under baseline c onditions (0.5 Hz, 1.5 mmol/L [Ca2+](o), 25 degreesC). The rates of relengt hening and decay in [Ca2+](i) were faster in TG than in WT 7-week AS myocyt es. In myocytes from WT 7-week AS compared with sham-operated WT mice, cont ractile reserve in response to rapid pacing was depressed with impaired aug mentation of both peak-systolic [Ca2+](i) and the SR Ca2+ load. In contrast , contractile reserve and the capacity to augment SR Ca2+ load were maintai ned in TG 7-week AS mice. SERCA2a protein levels were depressed in WT 7-wee k AS mice, but were preserved in TG 7-week AS mice. These data suggest that defective SR Ca2+ loading contributes to the onset of contractile failure in animals with chronic pressure overload.