Reactivity of MEST-1 (antigalactofuranose) with Trypanosoma cruzi, glycosylinositol phosphorylceramides (GIPCs): Immunolocalization of GIPCs in acidic vesicles of epimastigotes

Authors
Suzuki, E Mortara, RA Takahashi, HK Straus, AH
Citation
E. Suzuki et al., Reactivity of MEST-1 (antigalactofuranose) with Trypanosoma cruzi, glycosylinositol phosphorylceramides (GIPCs): Immunolocalization of GIPCs in acidic vesicles of epimastigotes, CL DIAG LAB, 8(5), 2001, pp. 1031-1035
Citations number
19
Categorie Soggetti
Immunology
Journal title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
ISSN journal
1071412X → ACNP
Volume
8
Issue
5
Year of publication
2001
Pages
1031 - 1035
Database
ISI
SICI code
1071-412X(200109)8:5<1031:ROM(WT>2.0.ZU;2-A
Abstract
Using confocal microscopy, MEST-1-positive immunofluorescence was observed within various Trypanosoma cruzi forms, except in cell-derived trypomastigo tes. Glycosylinositol phosphorylceramides were identified by thin-layer chr omatography immunostaining as the antigens recognized by MEST-1 in these pa rasites. In epimastigotes, labeling of MEST-1 coincided with acidic vesicle s, indicating an internal localization of these glycoconjugates.