Identification of peroxisomal membrane proteins of Saccharomyces cerevisiae by mass spectrometry

The identification of peroxisomal membrane proteins is very important to un derstand the import mechanisms of substrates and proteins into these organe lles and the pathogenesis of human peroxisomal disorders like the Zellweger Syndrom. Peroxisomal membrane proteins were identified after separation by gel electrophoresis, tryptic digestion and mass spectrometric analysis. Us ing matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS ) and nanoliquid chromatography-electrospray ionization-mass spectrometry ( LC-ESI-MS), it was possible to identify 45 proteins of isolated yeast perox isomal membranes.