Analysis of three marine fish cell lines by RAPD assay

We tested the applicability of the random amplified polymorphic deoxyribonu cleic acid (RAPD) analysis for identification of three marine fish cell lin es FG. SPH, and RSBK and as a possible tool to detect cross-contamination. Sixth commercial 10-mer RAPD primers were tested on the cell lines and on s amples collected from individual fish. The results obtained showed that the cell lines could be identified to the correspondent species on the basis o f identical Patterns produced by 35-48% of the primers tested. the total me an similarity indices for cell lines versus correspondent species of indivi dual fish ranged from 0.825 to 0.851. indicating the existence of genetic v ariation in these cell lines in relation to the species of their origin. Al so, four primers, which gave a monomorphic hand pattern within species/line , but different among the species/line, were obtained. These primers can be useful for identification of these cell lines arid for characterization of the genetic variation of these cell lines in relation to the species of th eir origin. This supported the use of RAPD analysis as an effective tool in species identification and cross-contamination test among different cell l ines.