IMMOBILIZED ASPERGILLUS-ORYZAE PROTEASE-CATALYZED FORMATION OF PEPTIDE-BONDS IN ORGANIC-SOLVENT

Immobilized Aspergillus oryzae protease (AOP) catalyzed the formation of peptide bonds between N-protected amino acids and amino acid eaters or amides in ethyl acetate. The influences of pH and reaction time on the coupling of Boc-L-Tyr and Gly-NH2 were studied. The optimal react ion condition for this enzyme catalyzed synthesis of Boc-L-Phe-Gly-NH2 (98.66%) was at pH 5.5 and a duration of 48 hours.