Residue aspartate-147 from the third transmembrane region of Na+/H+ antiporter NhaB of Vibrio alginolyticus plays a role in its activity

Authors
Nakamura, T Fujisaki, Y Enomoto, H Nakayama, Y Takabe, T Yamaguchi, N Uozumi, N
Citation
T. Nakamura et al., Residue aspartate-147 from the third transmembrane region of Na+/H+ antiporter NhaB of Vibrio alginolyticus plays a role in its activity, J BACT, 183(19), 2001, pp. 5762-5767
Citations number
23
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
183
Issue
19
Year of publication
2001
Pages
5762 - 5767
Database
ISI
SICI code
0021-9193(200110)183:19<5762:RAFTTT>2.0.ZU;2-F
Abstract
NhaB is a bacterial Na+/H+ antiporter with unique topology. The pH dependen ce of NhaB from Vibrio alginolyticus differs from that of the Escherichia c oli NhaB homolog. Replacement of Asp-147 with Gin made high H+ concentratio ns a requirement for the NhaB activity. Replacement of Asp-147 with neutral amino acids inactivated NhaB.