Rate of transmission and endogenous origin of Candida albicans and Candidaglabrato on adult intensive care units studied by pulsed field gel electrophoresis

We determined the relative roles of endogenous origin and patient-to-patien t transmission in Candida colonization of patients on adult intensive care units (ICU). A total of 48 Candida albicans and 18 Candida glabrata strains from various clinical samples of 28 long-term patients, hospitalized in tw o neurological ICUs between April and June 1999, were typed using pulsed fi eld gel electrophoresis (PFGE). Three patients were co-colonized by both C. albicans and C. glabrata strains. Twenty-four C. albicans and 17 C. glabra ta karyotypes were defined. The colonization was found to be polyclonal in six C. albicans and five C. glabrata patients. Twenty-six patients (93%) ca rried strains, which were not detected in other patients hospitalized at th e same time, i.e. they were colonized by unique C. albicans and C. glabrata strains. Only two patients, who were hospitalized during the same period o f time, although in different rooms of the same ICU, shared strains with an identical PFGE type, indicating possible patient-to-patient transmission. Patient-to-patient transmission of yeasts played a minor role on these ICUs . (C) 2001 The Hospital Infection Society.