E. Meffre et al., Circulating human B cells that express surrogate light chains display a unique antibody repertoire, J IMMUNOL, 167(4), 2001, pp. 2151-2156
Circulating human B cells that coexpress V-preB and conventional L chains (
V-preB(+)L(+) B cells) are a recently described subset of B cells that expr
ess Abs with features of self-reactivity. Initial analysis of V-preB(+)L(+)
B cells was limited to Ig-kappa and to the small, underused V(H)5 family.
To determine whether Abs commonly expressed by V-preB(+)L(+) B cells show s
imilar features, we analyzed Ig H chains from three highly expressed V-H fa
milies, V(H)1, V(H)3, and V(H)4, and Ig-lambda. We find that V(H)1 and V(H)
3 Abs expressed by V-preB(+)L(+) B cells resemble V(H)5 in that they displa
y increased J(H)6 use, long CDR3s, and an increased frequency of D-D fusion
s. Abs in all three of these V-H families also show skewed D reading frame
use resulting in predominance of hydrophobic amino acids, which are counter
selected in conventional B cells. Like Ig-kappa genes, the Ig-kappa genes i
n V-preB(+)L(+) B cells show long CDR3s, but they differ from Ig-kappa gene
s in that they display no evidence of receptor editing. We conclude that a
large number of H and L chain Abs expressed by V-preB(+)L(+) B cells displa
y features associated with self-reactive Abs.