S. Kaplan et al., A simple technique to measure the movements of the microscope stage along the x and y axes for stereological methods, J MICROSC O, 203, 2001, pp. 321-325
Measurements of microscope stage movements in the x and y directions are of
importance for some stereological methods such as the optical disector and
optical fractionator. The length of stage movements can be measured with g
reat precision and accuracy using a suitable motorized stage, which is gene
rally a computer-assisted instrument. This type of equipment is generally t
oo expensive for and not readily available in many laboratories. This paper
describes a simple method to measure the movements of the microscope stage
along the x and y directions, which can be used for purposes such as syste
matic uniform random sampling. It needs a microscope attachment consisting
of two dial indicators; one of them is used to measure the amount of stage
movement along the x-axis and the other measures the amount of movement alo
ng the y-axis. Movements of the stage on the micrometre-scale can be measur
ed easily using this device.