Suppression of the transcriptional activity and DNA binding of nuclear factor-kappa B by a paraneoplastic cerebellar degeneration-associated antigen

Paraneoplastic cerebellar degeneration (PCD) associated with gynecological malignancies is a disorder in which an autoimmune mechanism has been sugges ted. and both antibody- and cell-mediated immune responses exist against pc d17/cdr2, a neural protein expressed in cerebellar Purkinje neurons and bra instem neurons. In this report, we describe that pcd17 can suppress the bas al or activated NF-kappaB-dependent transcriptional activity in a co-transf ection study. The DNA binding of constitutive NF-kappaB complexes decreased in the nucleus of TNF-alpha -stimulated neuroblastoma cells. though pcd17 does not bind to classical NF-kappaB consensus site. These data indicate th at pcd17 is a potential repressor for NF-kappaB-dependent gene transcriptio n in neurons. (C) 2001 Elsevier Science B.V. All rights reserved.