Coordinately regulated expression of FAT/CD36 and FACS1 in rat skeletal muscle

Citation
Jjfp. Luiken et al., Coordinately regulated expression of FAT/CD36 and FACS1 in rat skeletal muscle, MOL C BIOCH, 223(1-2), 2001, pp. 61-69
Citations number
45
Categorie Soggetti
Cell & Developmental Biology
Journal title
MOLECULAR AND CELLULAR BIOCHEMISTRY
ISSN journal
03008177 → ACNP
Volume
223
Issue
1-2
Year of publication
2001
Pages
61 - 69
Database
ISI
SICI code
0300-8177(200107)223:1-2<61:CREOFA>2.0.ZU;2-1
Abstract
Protein-mediated fatty acid uptake and intracellular fatty acid activation are key steps in fatty acid metabolism in muscle.We have examined (a) the a bundance of fatty acid translocase (FAT/CD36) mRNA (a fatty acid transporte r) and long-chain acyl CoA synthetase (FACS1) mRNA in metabolically heterog eneous muscles (soleus (SOL), red (RG) and white gastrocnemius (WG)), and ( b) whether FAT/CD36 and FACS1 mRNAs were coordinately upregulated in red (R TA) and white tibialis muscles (WTA) that had been chronically stimulated f or varying periods of time (0.25, 1, 6 and 24 h/day) for 7 days. FAT/CD36 m RNA and FACS1 mRNA abundance were scaled with (a) the oxidative capacity of muscle (SOL > RG > WG) (p < 0.05), (b) the rates of fatty acid oxidation i n red and white muscles, and (c) fatty acid uptake by sarcolemmal vesicles, derived from red and white muscles. In chronically stimulated muscles (RTA and WTA), FAT/CD36 mRNA and FACS1 mRNA were up-regulated in relation to th e quantity of muscle contractile activity (p < 0.05). FAT/CD36 mRNA and FAC S1 mRNA up-regulation was highly correlated (r = 0.98). The coordinated exp ression of FAT/CD36 and FACS is likely a functional adaptive response to fa cilitate a greater rate of fatty acid activation in response to a greater r ate of fatty acid transport, either among different types of muscles or in muscles in which capacity for fatty acid metabolism has been enhanced.