Scanning transmission ion microscopy (STIM) imaging of living cultured cell
s has been carried out using a proton external-beam with the nuclear microp
robe of Bordeaux-Gradignan. STIM could be performed in air atmosphere after
passage of a focused proton beam through a 150 nm thick silicon nitride wi
ndow. Energy loss STIM images were obtained with a spatial resolution in th
e micrometer range and enabled the identification of sub-cellular ultrastru
ctures. (C) 2001 Elsevier Science B.V. All rights reserved.