Ea. Palmer et al., A yeast expression vector and leucine selection in Escherichia coli to aidin the identification of novel genes, PLASMID, 46(1), 2001, pp. 57-59
The complete sequencing of the Saccharomyces cerevisiae genome provides a p
owerful toot for studying and elucidating essential cellular processes. To
aid in the application of this resource to investigations into the molecula
r mechanisms of endoplasmic reticulum-associated protein degradation, a sim
ple procedure was designed to generate a unique 2-mum LEU2-selectable yeast
expression vector. Putative genes easily inserted into this vector are und
er control of the ADH1 promoter and transcription terminator sequences. Fur
thermore, a LEU2 selection in both yeast and Escherichia coli was used to a
llow the isolation of underrepresented plasmid from a pool of multiple vect
ors. Together, these advances in technology will be useful in the systemati
c analysis of novel yeast gene function, (C) 2001 Academic Press.