We determined the nucleotide sequence of the enolase (eno) gene of Tre
ponema pallidum, the noncultivable agent of syphilis. The deduced amin
o acid sequence of T. pallidum enolase (Eno) is 432 amino acids long w
ith a predicted molecular mass of 46.7 kDa. The Eno amino acid sequenc
e has a high degree of homology to the amino acid sequences of prokary
otic and eukaryotic Eno. This is the first eno sequence reported for a
bacterium in the order Spirochaetales.