Immobilization of urease in conducting thiophene-capped poly(methyl methacrylate)/pyrrole matrices

Authors
Alkan, S Toppare, L Bakir, U Yagci, Y
Citation
S. Alkan et al., Immobilization of urease in conducting thiophene-capped poly(methyl methacrylate)/pyrrole matrices, SYNTH METAL, 123(1), 2001, pp. 95-99
Citations number
21
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science
Journal title
SYNTHETIC METALS
ISSN journal
03796779 → ACNP
Volume
123
Issue
1
Year of publication
2001
Pages
95 - 99
Database
ISI
SICI code
0379-6779(20010822)123:1<95:IOUICT>2.0.ZU;2-B
Abstract
Urease was immobilized in conducting polypyrrole and block copolymers of th iophene-capped poly(methyl methacrylate) matrices by electropolymerization. Immobilization of the enzyme was achieved by application of 1.0 V constant potential in a solution of 0.02 M pyrrole, 2 mg/ml urease, 0.5 mg/ml suppo rting electrolyte. The optimum immobilization conditions, electrolysis time , electrolysis medium, enzyme concentration during electrolysis, type of su pporting electrolyte were determined. The kinetic behaviors of free and imm obilized urease were determined. (C) 2001 Elsevier Science B.V. All rights reserved.