Effect of dl-praeruptorin A on ATP sensitive potassium channels in human cortical neurons

AIM: To investigate the effect of dl-praeruptorin A (Pd-Ia) on ATP sensitiv e potassium channels (K-ATP channel) in human cortical neurons. METHODS; Us ing standard whole cell recording method. Cell membranes were held at - 40 mV, commanding potential was - 30 to + 100 mV and duration was 600 ms. RESU LTS - Pd-Ia activated K-ATP channels in human cortical neurons in a concent ration-dependent manner. After consecutive perfusion with external solution containing Pd-Ia 0.001, 0.01, 0.1, and I mu mol/L, currents increased from control (0.9 +/- 0.4) nA to (1.0 +/- 0.4) nA, (1.1 +/- 0.4) nA, (1.2 +/- 0 .4) nA, and (1.3 +/- 0.4) nA (P < 0. 05 or P < 0.01, n = 5) respectively. T hen the current decreased to (0.90 +/- 0.37) nA (P < 0.01, n = 5) after was hout with glibenclamide 10 mu mol/L The increscent part of the currents cou ld nearly be inhibited by specific K-ATP channel inhibitor. CONCLUSION: Pd- Ia could open K-ATP channel and it is a kind of potassium channel opener.