IMPROVED DETECTION OF POTATO LEAFROLL LUTEOVIRUS IN LEAVES AND TUBERSWITH A DIGOXIGENIN-LABELED CRNA PROBE

Authors
LOEBENSTEIN G AKAD F FILATOV V SADVAKASOVA G MANADILOVA A BAKELMAN H TEVEROVSKY E LACHMANN O DAVID A
Citation
G. Loebenstein et al., IMPROVED DETECTION OF POTATO LEAFROLL LUTEOVIRUS IN LEAVES AND TUBERSWITH A DIGOXIGENIN-LABELED CRNA PROBE, Plant disease, 81(5), 1997, pp. 489-491
Citations number
13
Categorie Soggetti
Plant Sciences
Journal title
Plant disease → ACNP
ISSN journal
01912917
Volume
81
Issue
5
Year of publication
1997
Pages
489 - 491
Database
ISI
SICI code
0191-2917(1997)81:5<489:IDOPLL>2.0.ZU;2-U
Abstract
A digoxigenin-labeled cRNA probe of approximately 2,100 bp was more th an 2,000 times more sensitive in detecting potato leafroll virus (PLRV ) in leaf extracts of Datura stramonium, Physalis floridana, and potat oes than enzyme-linked immunosorbent assay (ELISA). The limit of detec ting PLRV with the probe was 1 pg/ml compared with 2 ng/ml by ELISA. T he probe detected PLRV easily in dormant tuber tissues at dilutions of up to 1:100. There was no background reaction with healthy extracts. No reactions were observed between the probe and potato X potexvirus o r potato Y potyvirus.