T. Katagiri et al., Construction and characterization of BAC libraries for three fish species;rainbow trout, carp and tilapia, ANIM GENET, 32(4), 2001, pp. 200-204
Bacterial artificial chromosome (BAC) libraries are important tools for gen
omic research. We have constructed seven genomic BAC libraries from three f
ish species, rainbow trout (Oncorhynchus mykiss), carp (Cyprinus carpio) an
d tilapia (Oreochromis niloticus). The two rainbow trout BAC libraries have
average insert sizes of 58 and 110 kb. The average size of inserts in the
carp BAC library is 160 kb. The average insert sizes of the four tilapia BA
C libraries are 65, 105, 145 and 194 kb, respectively. These libraries repr
esent good coverage of each genome (2-64 x coverage). The libraries can be
screened by conventional colony hybridization and provide a starting point
for the construction of high-density filtres or polymerase chain reaction (
PCR) screening approaches. These BAC libraries will facilitate the position
al cloning of quantitative trait loci (QTLs) for a variety of economically
important traits in these species.