The effects of pokeweed mitogen (PWM) and phytohemagglutinin (PHA) on bovine oocyte maturation and embryo development in vitro

Authors
Wang, S Panter, KE Evans, RC Bunch, TD
Citation
S. Wang et al., The effects of pokeweed mitogen (PWM) and phytohemagglutinin (PHA) on bovine oocyte maturation and embryo development in vitro, ANIM REPROD, 67(3-4), 2001, pp. 215-220
Citations number
17
Categorie Soggetti
Animal Sciences
Journal title
ANIMAL REPRODUCTION SCIENCE
ISSN journal
03784320 → ACNP
Volume
67
Issue
3-4
Year of publication
2001
Pages
215 - 220
Database
ISI
SICI code
0378-4320(20010915)67:3-4<215:TEOPM(>2.0.ZU;2-O
Abstract
The effects of two commonly used cell culture mitogens, pokeweed (PWM) and phytohemagglutinin (PHA) on bovine oocyte maturation in vitro (IVM) and pre implantation embryo development in vitro were evaluated by randomized compl ete block experimental design with three treatments, Effects were measured by quantifying subsequent embryo development. Oocyte maturation was adverse ly affected by PWM-containing medium as indicated by a decrease in cleavage rate and subsequent embryo development to morula and blastocyst stages. Em bryo developmental competence was also adversely affected by PWM. Developme nt in PHA-containing medium was significantly better (P < 0.05) than in the PWM treatment. although there was no difference (P > 0.05) when compared t o Control. We conclude that there are no beneficial effects in adding mitog enic agents to culture medium to enhance in vitro embryo production and dev elopment. (C) 2001 Elsevier Science B.V. All rights reserved.