Measurement of pyruvate in blood by high-performance liquid chromatographywith fluorescence detection

Authors
Wulkan, RW Verwers, R Neele, M Mantel, MJ
Citation
Rw. Wulkan et al., Measurement of pyruvate in blood by high-performance liquid chromatographywith fluorescence detection, ANN CLIN BI, 38, 2001, pp. 554-558
Citations number
23
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
ANNALS OF CLINICAL BIOCHEMISTRY
ISSN journal
00045632 → ACNP
Volume
38
Year of publication
2001
Part
5
Pages
554 - 558
Database
ISI
SICI code
0004-5632(200109)38:<554:MOPIBB>2.0.ZU;2-4
Abstract
Accurate measurement of pyruvate is particularly important in the detection of mitochondrial enzyme disorders. We describe a simple highperformance li quid chromatography assay for pyruvate with derivatization and fluorescence detection. Between-run variation is below 4.5% and the method is linear be tween 1 and 1200 mu mol/L. The mean recovery is 101%. After sample pretreat ment, the calculated pyruvate concentration proved to be stable for 15 days when samples were kept at -20 degreesC.