AMPHOTERICIN-B RESISTANCE TESTING OF CANDIDA SPP - A COMPARISON OF METHODS

Several methods of susceptibility testing were compared for their abil ity to detect amphotericin B resistance among yeast isolates, includin g isolates known to be amphotericin-B-resistant in an animal model. A microtitre MIC method using antibiotic medium no. 3 and Etest strips w ere able to discriminate susceptible and resistant isolates. A disc su sceptibility test was also able to identify resistant isolates and thi s method may be suitable for routine use as a screening test. A survey of resistance in 644 clinical isolates showed that the incidence of e levated MICs (greater than or equal to 0.25 mg/L) was only 1.2%.