Protein kinase D interacts with Golgi via its cysteine-rich domain

Protein kinase D (PKD)/protein kinase C mu is a serine/threonine protein ki nase that has been localized in the cytosol and in several intracellular co mpartments including Golgi, mitochondria and plasma membrane. Using real ti me imaging of fluorescent protein (GFP)-tagged PKD, we have found that the accumulation of PKD in the Golgi compartment, following a temperature shift from 37 to 20 degreesC, was mediated by the cysteine-rich domain (CRD) of PKD. The CRD of PKD also mediates its interaction with the plasma membrane, further supporting the conclusion that the CRD of PKD may act as a subcell ular localization Signal. (C) 2001 Academic Press.