Rm. Abdeev et al., Exploring the properties of thermostable Clostridium thermocellum cellulase CelE for the purpose of its expression in plants, BIOCHEM-MOS, 66(7), 2001, pp. 808-813
The main properties(pH and temperature range, stability, substrate specific
ity) of the modified cellulase CelE (endo-beta -1,4-glucanase) from Clostri
dium thermocellum have been analyzed with the goal of its expression in pla
nts. The modified enzyme is similar to plant cellulases. Deletions in the N
-terminus of the enzyme do not affect its biochemical properties. Based on
the present investigation, we conclude that the modified beta -1,4-glucanas
e CelEM1,when expressed in plants, will be a good model to study the role o
f cellulases in plants.