C. Hoppe et al., A novel multilocus genotyping assay to identify genetic predictors of stroke in sickle cell anaemia, BR J HAEM, 114(3), 2001, pp. 718-720
We describe a novel multilocus genotyping assay permitting simultaneous ide
ntification of 60 candidate markers for stroke in sickle cell anaemia (SCA)
. Based on cerebral magnetic resonance imaging (MRI), 69 patients were divi
ded into stroke and control groups. The variant allele, CBS 278thr, showed
protection from stroke, whereas the apoE3 allele showed a trend towards ass
ociation with increased stroke risk. Several other variant alleles [TNF alp
ha (-308)A, CETP (-628)A, apoCIII (-641)A] showed a trend towards significa
nt associations with stroke risk. These preliminary results on a small grou
p of patients suggest that a multilocus genotyping assay may be valuable in
identifying genes that increase the risk of stroke in SCA.