C. Kawasaki et al., Ketamine isomers suppress superantigen-induced proinflammatory cytokine production in human whole blood, CAN J ANAES, 48(8), 2001, pp. 819-823
Citations number
24
Categorie Soggetti
Aneshtesia & Intensive Care","Medical Research Diagnosis & Treatment
Journal title
CANADIAN JOURNAL OF ANAESTHESIA-JOURNAL CANADIEN D ANESTHESIE
Purpose: To investigate the efficacy of S(+)-ketamine and R(-)-ketamine on
staphylococcal enterotoxin B (SEB)-induced tumour necrosis factor (TNF)-, i
nterleukin (IL)-6, and IL-8 production in human whole blood in vitro.
Methods: After Ethics Committee approval and informed consent, blood sample
s were obtained from ten healthy volunteers and diluted with five volumes o
f RPMI 1640. After adding different doses of ketamine isomers (0-1000 muM),
the blood was stimulated with SEB (10 ng.mL(-1)). After a six-hour incubat
ion period, the plasma TNF- activity was determined by the L929 cell cytoto
xic assay and IL-6 and IL-8 concentrations were measured using an enzyme-li
nked immunoassay.
Results: Ketamine isomers significantly suppressed SEB-induced TNF- product
ion at concentrations exceeding 50 muM. Ketamine isomers at concentrations
exceeding 100 muM also significantly suppressed SEB-induced IL-6 production
. Furthermore, ketamine isomers at concentrations exceeding 500 muM signifi
cantly suppressed SEB-induced IL-8 production. There were no significant di
fferences between the suppressive effects of S(+)-ketamine and R(-)-ketamin
e on SEB-induced proinflammatory cytokine production.
Conclusion: This study demonstrated that ketamine isomers suppressed SEB-in
duced TNF-, IL-6, and IL-8 production in human whole blood.