Xf. Bai et al., On the site and mode of antigen presentation for the initiation of clonal expansion of CD8 T cells specific for a natural tumor antigen, CANCER RES, 61(18), 2001, pp. 6860-6867
Because of the low frequency of antigen-specific T cells, early events in t
he activation of tumor-specific T cells in vivo have not been well characte
rized. There is still no direct documentation on where the clonal expansion
begins and how tumor antigens are presented to the host CD8 T cells to ini
tiate it. Here we used transgenic T cells specific for a natural tumor anti
gen PIA to evaluate the kinetics. location, and modes of antigen presentati
on for initiating CTL response in vivo. Our results demonstrate that the in
itial activation of PIA-specific T cells takes place in the lymphoid organs
. The activated T cells then migrate into tumors, where they undergo accele
rated division and acquire distinct activation markers. The site of initiat
ion cannot be altered by either local expression of costimulatory molecules
or by intratumor injection of naive T cells. Moreover, using genetic model
s that allow only one mode of antigen presentation, we show here that both
cross-presentation of PIA by the host antigen-presenting cells. and direct
antigen presentation and costimulation by the tumor cells are sufficient to
initiate rapid T cell-clonal expansion in the lymphoid organ. These result
s provide direct evidence for two fundamental assumptions on the mechanisms
of T-cell activation in vivo.