SOLUBILIZING COELENTERAZINE IN WATER WITH HYDROXYPROPYL-BETA-CYCLODEXTRIN

Authors
TERANISHI K SHIMOMURA O
Citation
K. Teranishi et O. Shimomura, SOLUBILIZING COELENTERAZINE IN WATER WITH HYDROXYPROPYL-BETA-CYCLODEXTRIN, Bioscience, biotechnology, and biochemistry, 61(7), 1997, pp. 1219-1220
Citations number
19
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
7
Year of publication
1997
Pages
1219 - 1220
Database
ISI
SICI code
0916-8451(1997)61:7<1219:SCIWWH>2.0.ZU;2-X
Abstract
Coelenterazine is poorly soluble in water except at alkaline pH values that promote auto-oxidation. The solubility is markedly increased by adding hydroxypropyl-beta-cyclodextrin in a neutral buffer solution, t he solubilization being most effective in the pH range of 6.5 to 7.0. The concentration of coelenterazine dissolved in a pH 7.0 buffer conta ining 50 mM hydroxypropyl-beta-cyclodextrin was about 280-fold higher than that without the additive.