M. Mokotoff et al., EVALUATION OF LAMININ PEPTIDE-FRAGMENTS LABELED WITH IN-111 FOR THE POTENTIAL IMAGING OF MALIGNANT-TUMORS, The journal of peptide research, 49(6), 1997, pp. 510-516
The laminin peptide fragments GYIGSR-NH2 and CDPGYIGSR-NH2 are known t
o bind to a 67-kDa laminin receptor. This receptor is understood to be
expressed at higher than normal levels in malignant tumor cells, part
icularly those of breast and colon carcinomas. Peptides DTPA-GYIGSR-NH
2 (1), DTPA-(GYIGSR-NH2)(2) (2), DTPA-CDPGYIGSR-NH2 (3), DTPA-(CDPGYIG
SR-NH2)(2) (4), and negative control DTPA-GAGAGA-NH2 (5) were prepared
by solid-phase peptide synthesis. AU five DTPA-conjugated peptides we
re subsequently radiolabeled with In-111 and their tissue distribution
evaluated in mice bearing C3H tumors, In-111-3 and In-111-4 showed th
e highest specific tumor localization. These preliminary data support
father study of radiolabeled peptide fragments fur the potential detec
tion of malignant tumors of the breast and other organs. (C) Munksgaar
d 1997.