The correlation of lipid peroxidation with release of alkanes (RH) is consi
dered a noninvasive method for the in vivo evaluation of oxidative stress.
The formation of RH is believed to reflect a lipid hydroperoxide (LOOH)-dep
endent generation of alkoxyl radicals (LO.) that undergo, beta -scission wi
th release of alkyl radicals (R-.). Alternatively, R-. could be spin-trappe
d with a nitrone before the formation of RH and analyzed by ESR. Extracts f
rom the liver and lung of CCl4- and asbestos-treated rats that were previou
sly loaded with nitrones exhibited ESR spectra suggesting the formation of
iso-propyl, n-butyl, ethyl, and pentyl radical-derived nitroxides. In biolo
gical systems, various nitroxides with indistinguishable ESR spectra could
be formed. Hence, experiments with N-tert-butyl-alpha -phenylnitrone (PBN)
for spin trapping of R-. were carried out in which the nitroxides formed we
re separated and analyzed by HPLC with electrochemical detection (EC). The
C1-5 homologous series of PBN nitroxides and hydroxylamines were synthesize
d, characterized by ESR, GC-MS, and HPLC-EC, and used as HPLC standards. Fo
r in vivo generation and spin trapping of R-., rats were loaded with CCl4 a
nd PBN. The HPLC-EC chromatograms of liver extracts from CCl4-treated rats
demonstrated the formation of both the nitroxide and hydroxylamine forms of
PBN/(CCl3)-C-., as well as the formation of a series of unidentified PBN n
itroxides and hydroxylamines. However, formation of PBN adducts with retent
ion times similar to these of the PBN/C2-5 derivatives was not observed. In
conclusion, we could not correlate the production of PBN-detectable alkyl
radicals with the reported CCl4-dependent production Of C1-5 alkanes. We sp
eculate that the major reason for this is the low steady-state concentratio
ns of R-. produced because only a small fraction of LO. undergo,beta -sciss
ion to release R-..