In order to evaluate the bacterial and endotoxin contamination in the
dialysis fluids of our pediatric center and the effectiveness of chlor
ine dioxide (CD) compared with a conventional method, (1) deionized wa
ter, (2) dialysate fluid, (3) basic concentrate, and (4) acid concentr
ate were tested in 4 dialysis machines. Monitor sterilization was made
using CD in protocol A and sodium hypochlorite/acetic acid in protoco
l B. Once every 2 weeks the deionized water set of distribution was ro
utinely disinfected with peracetic acid. Each protocol lasted 1 month
and the samples were taken, under aseptic conditions, on the 15th, 22n
d and 27th day. All samples, at all stages of the study, showed an end
otoxin concentration below the limits recommended by the Canadian Stan
dard Association. Fifty-nine out of 72 samples in A and 62 out of 72 s
amples in B showed a bacterial count within the range recommended by t
he Association for the Advancement of Medical Instrumentation. The dat
a show that both protocols produced the same results. However, protoco
l A is to be preferred for its simultaneous disinfecting-cleaning and
descaling activity which proves time-saving.