Evaluation of ribotyping for characterization and identification of Enterococcus haemoperoxidus and Enterococcus moraviensis strains

Seven Enterococcus moraviensis and 16 Enterococcus haemoperoxidus as well a s nine reference cultures of other enterococcal species obtained from the C zech Collection of Microorganisms were characterized using ribotyping with EcoRI and HindIII in the present work. The ribopatterns obtained by both re striction enzymes clearly distinguished all E moraviensis and E. haemoperox idus strains from the other enterococci (E faecalis, E faecium, E avium, E raffinosus, E. pseudoavium, E. malodoratus) and they differentiated both sp ecies from each other as well. Although all strains were isolated from diff erent sampling sites, many strains shared the same band patterns. E. moravi ensis formed four ribogroups using EcoRI and two ribogroups using HindIII r estriction enzyme. E haemoperoxidus gave six different patterns with EcoRI and five using the HindIII restriction enzyme. (C) 2001 Federation of Europ ean Microbiological Societies. Published by Elsevier Science B.V. All right s reserved.