Expression of the Pasteurella multocida ompH gene is negatively regulated by the Fur protein

The fur gene of Pasteurella multocida has been cloned by complementation of an Escherichia coli fur mutant. The P. multocida fur gene, which encodes a predicted protein of 147 amino acids, displaying the highest identity (89% ) with the same protein of Haemophilus influenzae, is negatively regulated by its own product. By construction of a P. multocida fur mutant, it has be en demonstrated that the ompH gene, encoding a major structural protein of the outer membrane, presenting high antigenicity power, is negatively regul ated by iron and glucose. Furthermore, wild-type and fur-defective cells of P. multocida show the same level of virulence. (C) 2001 Federation of Euro pean Microbiological Societies. Published by Elsevier Science B.V. All righ ts reserved.