Yn. Parsons et al., A putative type III secretion gene cluster is widely distributed in the Burkholderia cepacia complex but absent from genomovar I, FEMS MICROB, 203(1), 2001, pp. 103-108
Using probes constructed from Ralstonia solanacearum and Burkholderia pseud
omallei, putative type III secretion (TTS) genes were identified in Burkhol
deria cepacia J2315 (genomovar III). A cosmid clone containing DNA with hom
ology to five TTS genes was sub-cloned and regions were sequenced in order
to design oligonucleotides for polymerase chain reaction assays. These indi
cated that two putative TTS genes (bcscQ and bcscV) were present in all mem
bers of the B. cepacia complex with the exception of strains from genomovar
I. Southern blot assays confirmed this observation, suggesting that the la
ck of a TTS gene cluster may define a major difference between B. cepacia g
enomovar I and other members of the B. cepacia complex, including genomovar
III. In contrast to TTS gene clusters in other bacteria, a Putative gene h
omologous to the virB1 gene of Brucella sids was located directly downstrea
m of bcscQR. (C) 2001 Federation of European Microbiological Societies. Pub
lished by Elsevier Science B.V. All rights reserved.